Rohini is an active Ph.D. member in our lab. Her research area is focused on mammalian and bacterial cell systems for diagnostic and drug delivery applications. In her first project, Rohini has worked in collaboration with Dr. Neetu Singh’s lab at the Centre for Biomedical Engineering (CBME) at IIT Delhi for developing a gold-peptide conjudate colloidal system to tackle bacterial infections that get internalized inside cancer cells. This study has entailed synthesis and characterization of the particles and analyzing their antibacterial activity on both kinds of cells.(1)
In another study, Rohini is developing a one-of-its kind bedside bioassay for rapid and early diagnosis of Gram-negative bacterial infections in a drop of blood. She uses peptide-conjugated gold nanoparticles which have specific affinity for a Gram-negative bacterial biomarker, endotoxin, whose concentration in the blood is the first to rise (< 2 h) after infection.
References:
- Jagtap P.*, Singh R.*, Deepika K., Sritharan V., Gupta S., ‘A flow through assay for rapid, bedside stratification of endotoxemia in critically ill patients: A pilot study’, Journal of Clinical Microbiology, (2018) (*Equal contribution)
- Singh R. and Gupta S., ‘Dual-functionality nanobioconjugates: A new tool for intracellular bacterial targeting in cancer?‘, Therapeutic Delivery, 9, 317-320 (2018)
- Singh R.*, Patil S.*, Singh N. and Gupta S., ‘Antimicrobial Activity of Polymyxin B Sulfate and Sushi Peptide Nanoparticle Bioconjugates on Gram-negative Bacteria’ Scientific Reports 7:5792, 1- 10 (2017) (*Equal contribution)
